Biology Professional Development
Biology Laboratory Experience: CRISPR/Cas9 Gene Editing
The CRISPR/Cas9 method of precise gene editing is fast, cheap, easy and fun! This course is designed for K-12 STEM teachers and anyone else who wants to learn how to use CRISPR/Cas9 to edit genes. The course is hands-on and will take place in a UCCS Biology teaching lab.
NBMS 6001: Biology Laboratory Experience- 2 Continuing Education Units, $600
Course Objectives for Non-Credit:
Learn about the biology of the CRISPR and practice basic laboratory techniques for CRISPR/Cas9 gene editing.
Create targeted gene mutations in yeast and practice techniques of light and fluoresence microscopy required to characterize the effects of your targeted gene mutations.
Optional Additional enrollment-
BIOL 5000: Special Topics in Biology- 2 Graduate Credits, additional $500
Course Objectives for Graduate Credit:
*Includes objectives listed above
Learn how the materials and methods of this course can be integrated into a K-12 course of your own.
- The graduate credit requires an additional assignment after the laboratory experience
Course Overview: Tuesday and Friday afternoons July 9, 2019 - August 2, 2019
July 9th, 1:00pm-5:00pm: Use the online CRISPR4P tool to design gRNAs that will target Cas9 to DNA checkpoint genes in yeast
July 12th,1:00pm-5:00pm: Use a high-fidelity polymerase chain reaction to clone gRNAs into a Cas9 plasmid.
July 16th, 1:00pm-4:00pm: Purify recombinant DNA and prepare transformation competent yeast and selection media.
July 19th, 1:00pm-4:00pm: Transform yeast and then plate on selection media.
*No class during the week of July 22nd, when yeast transformants need time to grow.*
July 30th, 1:00pm-4:00pm: Identify and isolate CRISPR/Cas9-targeted DNA damage checkpoint mutants.
August 2nd, 1:00pm-4:00pm: Use epifluorescence microscopy to analyze DNA checkpoint mutant phenotypes.
Biology Laboratory Experience Interest
Seating is limited! If you are interested in the Biology Laboratory Experience, RSVP your spot now!