Biology Professional Development

 

Biology Laboratory Experience: CRISPR/Cas9 Gene Editing

The CRISPR/Cas9 method of precise gene editing is fast, cheap, easy and fun! This course is designed for K-12 STEM teachers and anyone else who wants to learn how to use CRISPR/Cas9 to edit genes. The course is hands-on and will take place in a UCCS Biology teaching lab.

 

Biology

NBMS 6001: Biology Laboratory Experience- 2 Continuing Education Units, $600

Course Objectives for Non-Credit:

Learn about the biology of the CRISPR and practice basic laboratory techniques for CRISPR/Cas9 gene editing.

Create targeted gene mutations in yeast and practice techniques of light and fluoresence microscopy required to characterize the effects of your targeted gene mutations.

 

Optional Additional enrollment-

BIOL 5000: Special Topics in Biology- 2 Graduate Credits, additional $500

Course Objectives for Graduate Credit:

*Includes objectives listed above

Learn how the materials and methods of this course can be integrated into a K-12 course of your own.

  • The graduate credit requires an additional assignment after the laboratory experience

 

Course Overview: Tuesday and Friday afternoons July 9, 2019 - August 2, 2019

July 9th, 1:00pm-5:00pm: Use the online CRISPR4P tool to design gRNAs that will target Cas9 to DNA checkpoint genes in yeast

July 12th,1:00pm-5:00pm: Use a high-fidelity polymerase chain reaction to clone gRNAs into a Cas9 plasmid.

July 16th, 1:00pm-4:00pm: Purify recombinant DNA and prepare transformation competent yeast and selection media.

July 19th, 1:00pm-4:00pm: Transform yeast and then plate on selection media.

 *No class during the week of July 22nd, when yeast transformants need time to grow.*

July 30th, 1:00pm-4:00pm: Identify and isolate CRISPR/Cas9-targeted DNA damage checkpoint mutants.

August 2nd, 1:00pm-4:00pm: Use epifluorescence microscopy to analyze DNA checkpoint mutant phenotypes.

 

Biology Laboratory Experience Interest

Seating is limited! If you are interested in the Biology Laboratory Experience, RSVP your spot now!

 

Name(s) of the participant(s):
more items

 

E-Mail Address of the participant(s):
more items

 

Name of your School
more items